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Part:BBa_K4813008

Designed by: YuHin Lau   Group: iGEM23_HongKong-JSS   (2023-10-04)


20-bp 3' overlapping sequence for pUC19 EcoRI restriction site

In our project, we used this 3' overlapping sequence for HiFi assembly to clone our constructs into pUC19 vectors. This particular sequence consists of 20 base pairs, which is the recommended length suggested by the HiFi assembly kit manufacturer. By employing this 3' overlapping sequence, along with a 5' overlapping sequence (BBa_K4813007) , we successfully assembled our four verified constructs (BBa_K4813002, BBa_K4813004, BBa_K4813005, and BBa_K4813006) using the HiFi assembly method.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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